Several studies have been conducted on technological, pedagogical content knowledge and web-based education. In this study, the Technological Pedagogical Content Knowledge and Educational Use of Web Technologies (TPCK-W) were analyzed in addition to the self-efficacy and attitudes of 33 teachers from eight different branches carrying out their duties in 19 countries of the European Union (EU). In this study, the Technological Pedagogical Content Knowledge-Web (TPCK-W) Survey developed by Lee, Tsai, and Chan was used. The data obtained statistics software was analyzed using SPSS for Windows 17.0 statistics software. As a result of the analysis, it was revealed that TPCK-W self-efficacy of teachers carrying out their duties in EU countries was high and their attitudes were positive; and age, experience, and gender did not affect their TPCK-W self-efficacy and attitudes. Moreover, participants’ general web attitudes changed positively, depending on their web communication, web content, and pedagogical use of the web.